Active Railroads
- Burlington Northern – La Crosse Sub
- Burlington Northern – Marias Pass
- CB&Q – Chicago-Aurora-C&I
- Chicago, Milwaukee, St. Paul and Pacific
- CPKC Davenport-Muscatine, IA
- Great Northern Mesabi Division
- Lake Erie & Southern
- Michigan Central
- Midwest Rail
- Milwaukee Road
- Montana Northland
- Peoria Western
- Schnitzel Terminal Railroad
- South Omaha Terminal Railway
- Wisconsin & Upper Michigan
Upcoming Railroads
- Chicago & North Western – Waseca Sub
- Chicago, Burlington & Quincy – Zone 3 La Crosse
- The Twin Cities Terminal
- Wisconsin & Upper Michigan